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srf expression plasmid  (Addgene inc)


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    Structured Review

    Addgene inc srf expression plasmid
    Oligonucleotides and plasmids.
    Srf Expression Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/srf+expression+plasmid/pmc03445523-38-2-6?v=Addgene+inc
    Average 92 stars, based on 11 article reviews
    srf expression plasmid - by Bioz Stars, 2026-07
    92/100 stars

    Images

    1) Product Images from "Transcriptional Regulation of Flotillins by the Extracellularly Regulated Kinases and Retinoid X Receptor Complexes"

    Article Title: Transcriptional Regulation of Flotillins by the Extracellularly Regulated Kinases and Retinoid X Receptor Complexes

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0045514

    Oligonucleotides and plasmids.
    Figure Legend Snippet: Oligonucleotides and plasmids.

    Techniques Used: Luciferase, Plasmid Preparation, Expressing, Control, Dominant Negative Mutation



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    The <t>SRF-promoted</t> angiogenesis in CRC. (A) Western blotting assays for verifying SRF siRNA and expression vector in HCT-116 and HCT-8 cells. (B) qPCR assays for verifying SRF siRNA in HCT-116 and HCT-8 cells ( n =6). (C) ELISAs to investigate the effects of SRF silencing on VEGF secretion in HCT-116 and HCT-8 cells ( n =3). (D) MTT assays for investigating the effects of CM from SRF-knockdown HCT-116 and HCT-8 cells on the proliferation of HUVECs ( n =6). (E) Transwell assays to investigate the effects of CM on the migration of HUVECs ( n =2). (F) Transwell assays to investigate the effects of CM on the invasion of HUVECs ( n =2). (G) The effects of CM on the tube formation of HUVECs ( n =3). (H) The effects of CM from HCT-116 and HCT-8 cells transfected with SRSF3 siRNA and <t>SRF</t> <t>expression</t> plasmid on the tube formation of HUVECs ( n =3). Data represent mean ± SD. Significance was assessed by two-sided t test. *** P < 0.001; ** P < 0.01; ns, no significance.
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    Image Search Results


    The SRF-promoted angiogenesis in CRC. (A) Western blotting assays for verifying SRF siRNA and expression vector in HCT-116 and HCT-8 cells. (B) qPCR assays for verifying SRF siRNA in HCT-116 and HCT-8 cells ( n =6). (C) ELISAs to investigate the effects of SRF silencing on VEGF secretion in HCT-116 and HCT-8 cells ( n =3). (D) MTT assays for investigating the effects of CM from SRF-knockdown HCT-116 and HCT-8 cells on the proliferation of HUVECs ( n =6). (E) Transwell assays to investigate the effects of CM on the migration of HUVECs ( n =2). (F) Transwell assays to investigate the effects of CM on the invasion of HUVECs ( n =2). (G) The effects of CM on the tube formation of HUVECs ( n =3). (H) The effects of CM from HCT-116 and HCT-8 cells transfected with SRSF3 siRNA and SRF expression plasmid on the tube formation of HUVECs ( n =3). Data represent mean ± SD. Significance was assessed by two-sided t test. *** P < 0.001; ** P < 0.01; ns, no significance.

    Journal: Frontiers in Oncology

    Article Title: SRSF3 Promotes Angiogenesis in Colorectal Cancer by Splicing SRF

    doi: 10.3389/fonc.2022.810610

    Figure Lengend Snippet: The SRF-promoted angiogenesis in CRC. (A) Western blotting assays for verifying SRF siRNA and expression vector in HCT-116 and HCT-8 cells. (B) qPCR assays for verifying SRF siRNA in HCT-116 and HCT-8 cells ( n =6). (C) ELISAs to investigate the effects of SRF silencing on VEGF secretion in HCT-116 and HCT-8 cells ( n =3). (D) MTT assays for investigating the effects of CM from SRF-knockdown HCT-116 and HCT-8 cells on the proliferation of HUVECs ( n =6). (E) Transwell assays to investigate the effects of CM on the migration of HUVECs ( n =2). (F) Transwell assays to investigate the effects of CM on the invasion of HUVECs ( n =2). (G) The effects of CM on the tube formation of HUVECs ( n =3). (H) The effects of CM from HCT-116 and HCT-8 cells transfected with SRSF3 siRNA and SRF expression plasmid on the tube formation of HUVECs ( n =3). Data represent mean ± SD. Significance was assessed by two-sided t test. *** P < 0.001; ** P < 0.01; ns, no significance.

    Article Snippet: The human Flag-tagged SRF expression plasmid was cloned into the GV492 vector, which was synthesized by GeneChem.

    Techniques: Western Blot, Expressing, Plasmid Preparation, Migration, Transfection

    Oligonucleotides and plasmids.

    Journal: PLoS ONE

    Article Title: Transcriptional Regulation of Flotillins by the Extracellularly Regulated Kinases and Retinoid X Receptor Complexes

    doi: 10.1371/journal.pone.0045514

    Figure Lengend Snippet: Oligonucleotides and plasmids.

    Article Snippet: pCGN-SRF , SRF expression plasmid , Addgene 11977, .

    Techniques: Luciferase, Plasmid Preparation, Expressing, Control, Dominant Negative Mutation